Infection of cultured intestinal epithelial cells with severe acute respiratory syndrome coronavirus.
Identifieur interne : 005286 ( Main/Exploration ); précédent : 005285; suivant : 005287Infection of cultured intestinal epithelial cells with severe acute respiratory syndrome coronavirus.
Auteurs : J. Cinatl [Allemagne] ; G. Hoever ; B. Morgenstern ; W. Preiser ; J-U Vogel ; W-K Hofmann ; G. Bauer ; M. Michaelis ; H F Rabenau ; H W DoerrSource :
- Cellular and molecular life sciences : CMLS [ 1420-682X ] ; 2004.
Descripteurs français
- KwdFr :
- Cellules Caco-2, Cellules épithéliales (métabolisme), Cellules épithéliales (ultrastructure), Cellules épithéliales (virologie), Expression des gènes, Humains, Intestins (ultrastructure), Intestins (virologie), Microscopie électronique, Modèles biologiques, Muqueuse intestinale (métabolisme), Récepteurs viraux (métabolisme), Survie cellulaire, Séquençage par oligonucléotides en batterie, Virus du SRAS (croissance et développement), Virus du SRAS (métabolisme), Virus du SRAS (pathogénicité).
- MESH :
- croissance et développement : Virus du SRAS.
- métabolisme : Cellules épithéliales, Muqueuse intestinale, Récepteurs viraux, Virus du SRAS.
- pathogénicité : Virus du SRAS.
- virologie : Cellules épithéliales, Intestins.
- ultrastructure : Cellules Caco-2, Cellules épithéliales, Expression des gènes, Humains, Intestins, Microscopie électronique, Modèles biologiques, Survie cellulaire, Séquençage par oligonucléotides en batterie.
English descriptors
- KwdEn :
- Caco-2 Cells, Cell Survival, Epithelial Cells (metabolism), Epithelial Cells (ultrastructure), Epithelial Cells (virology), Gene Expression, Humans, Intestinal Mucosa (metabolism), Intestines (ultrastructure), Intestines (virology), Microscopy, Electron, Models, Biological, Oligonucleotide Array Sequence Analysis, Receptors, Virus (metabolism), SARS Virus (growth & development), SARS Virus (metabolism), SARS Virus (pathogenicity).
- MESH :
- chemical , metabolism : Receptors, Virus.
- growth & development : SARS Virus.
- metabolism : Epithelial Cells, Intestinal Mucosa, SARS Virus.
- pathogenicity : SARS Virus.
- ultrastructure : Epithelial Cells, Intestines.
- virology : Epithelial Cells, Intestines.
- Caco-2 Cells, Cell Survival, Gene Expression, Humans, Microscopy, Electron, Models, Biological, Oligonucleotide Array Sequence Analysis.
Abstract
To identify a model for the study of intestinal pathogenesis of severe acute respiratory syndrome (SARS) we tested the sensitivity of six human intestinal epithelial cell lines to infection with SARS coronavirus (SARS-CoV). In permissive cell lines, effects of SARS-CoV on cellular gene expression were analysed using high-density oligonucleotide arrays. Caco-2 and CL-14 cell lines were found to be highly permissive to SARS-CoV, due to the presence of angiotensin-converting enzyme 2 as a functional receptor. In both cell lines, SARS-CoV infection deregulated expression of cellular genes which may be important for the intestinal pathogenesis of SARS.
DOI: 10.1007/s00018-004-4222-9
PubMed: 15316659
Affiliations:
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Le document en format XML
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Michaelis</name></author><author><name sortKey="Rabenau, H F" sort="Rabenau, H F" uniqKey="Rabenau H" first="H F" last="Rabenau">H F Rabenau</name></author><author><name sortKey="Doerr, H W" sort="Doerr, H W" uniqKey="Doerr H" first="H W" last="Doerr">H W Doerr</name></author></analytic><series><title level="j">Cellular and molecular life sciences : CMLS</title><idno type="ISSN">1420-682X</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Caco-2 Cells</term><term>Cell Survival</term><term>Epithelial Cells (metabolism)</term><term>Epithelial Cells (ultrastructure)</term><term>Epithelial Cells (virology)</term><term>Gene Expression</term><term>Humans</term><term>Intestinal Mucosa (metabolism)</term><term>Intestines (ultrastructure)</term><term>Intestines (virology)</term><term>Microscopy, Electron</term><term>Models, Biological</term><term>Oligonucleotide Array Sequence Analysis</term><term>Receptors, Virus (metabolism)</term><term>SARS Virus (growth & development)</term><term>SARS Virus (metabolism)</term><term>SARS Virus (pathogenicity)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Cellules Caco-2</term><term>Cellules épithéliales (métabolisme)</term><term>Cellules épithéliales (ultrastructure)</term><term>Cellules épithéliales (virologie)</term><term>Expression des gènes</term><term>Humains</term><term>Intestins (ultrastructure)</term><term>Intestins (virologie)</term><term>Microscopie électronique</term><term>Modèles biologiques</term><term>Muqueuse intestinale (métabolisme)</term><term>Récepteurs viraux (métabolisme)</term><term>Survie cellulaire</term><term>Séquençage par oligonucléotides en batterie</term><term>Virus du SRAS (croissance et développement)</term><term>Virus du SRAS (métabolisme)</term><term>Virus du SRAS (pathogénicité)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Receptors, Virus</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Epithelial Cells</term><term>Intestinal Mucosa</term><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Cellules épithéliales</term><term>Muqueuse intestinale</term><term>Récepteurs viraux</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="en"><term>Epithelial Cells</term><term>Intestines</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Cellules épithéliales</term><term>Intestins</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Epithelial Cells</term><term>Intestines</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Caco-2 Cells</term><term>Cell Survival</term><term>Gene Expression</term><term>Humans</term><term>Microscopy, Electron</term><term>Models, Biological</term><term>Oligonucleotide Array Sequence Analysis</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="fr"><term>Cellules Caco-2</term><term>Cellules épithéliales</term><term>Expression des gènes</term><term>Humains</term><term>Intestins</term><term>Microscopie électronique</term><term>Modèles biologiques</term><term>Survie cellulaire</term><term>Séquençage par oligonucléotides en batterie</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">To identify a model for the study of intestinal pathogenesis of severe acute respiratory syndrome (SARS) we tested the sensitivity of six human intestinal epithelial cell lines to infection with SARS coronavirus (SARS-CoV). In permissive cell lines, effects of SARS-CoV on cellular gene expression were analysed using high-density oligonucleotide arrays. Caco-2 and CL-14 cell lines were found to be highly permissive to SARS-CoV, due to the presence of angiotensin-converting enzyme 2 as a functional receptor. In both cell lines, SARS-CoV infection deregulated expression of cellular genes which may be important for the intestinal pathogenesis of SARS.</div></front></TEI><affiliations><list><country><li>Allemagne</li></country><region><li>District de Darmstadt</li><li>Hesse (Land)</li></region><settlement><li>Francfort-sur-le-Main</li></settlement></list><tree><noCountry><name sortKey="Bauer, G" sort="Bauer, G" uniqKey="Bauer G" first="G" last="Bauer">G. Bauer</name><name sortKey="Doerr, H W" sort="Doerr, H W" uniqKey="Doerr H" first="H W" last="Doerr">H W Doerr</name><name sortKey="Hoever, G" sort="Hoever, G" uniqKey="Hoever G" first="G" last="Hoever">G. Hoever</name><name sortKey="Hofmann, W K" sort="Hofmann, W K" uniqKey="Hofmann W" first="W-K" last="Hofmann">W-K Hofmann</name><name sortKey="Michaelis, M" sort="Michaelis, M" uniqKey="Michaelis M" first="M" last="Michaelis">M. Michaelis</name><name sortKey="Morgenstern, B" sort="Morgenstern, B" uniqKey="Morgenstern B" first="B" last="Morgenstern">B. Morgenstern</name><name sortKey="Preiser, W" sort="Preiser, W" uniqKey="Preiser W" first="W" last="Preiser">W. Preiser</name><name sortKey="Rabenau, H F" sort="Rabenau, H F" uniqKey="Rabenau H" first="H F" last="Rabenau">H F Rabenau</name><name sortKey="Vogel, J U" sort="Vogel, J U" uniqKey="Vogel J" first="J-U" last="Vogel">J-U Vogel</name></noCountry><country name="Allemagne"><region name="Hesse (Land)"><name sortKey="Cinatl, J" sort="Cinatl, J" uniqKey="Cinatl J" first="J" last="Cinatl">J. Cinatl</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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